A rapid and extraction-free diagnostic platform that detects ASFV within 20 min.
sCRAM integrates a rapid nucleic acid release step (40◦C, 5 min) with a one-tube reaction combining Multienzyme Isothermal Rapid Amplification (MIRA) and a suboptimal PAM-mediated CRISPR/Cas12a system (37◦C, 15 min), enabling instrument-free visual readout via UV light or lateral flow strips (LFS).
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sCRAM integrates a rapid nucleic acid release step (40◦C, 5 min) with a one-tube reaction combining Multienzyme Isothermal Rapid Amplification (MIRA) and a suboptimal PAM-mediated CRISPR/Cas12a system (37◦C, 15 min), enabling instrument-free visual readout via UV light or lateral flow strips (LFS).
You can scan the QR code to view the original text.